N 5 Aminopentyl maleimide trifluoroacetate salt (2024)

Tomás J. Steeman, et al., bioRxiv - Molecular Biology 2023

Quote: ... PDS (pyridostatin trifluoroacetate salt, Sigma-Aldrich SML0678) was added at different concentrations (0 ...

Junxiong Mao, et al., bioRxiv - Biochemistry 2023

Quote: ... of fluorescein-5-maleimide (Invitrogen, F150) was added to 2 mg Ub mutants or K48-linked diUb variants ...

Wei-Yun Wholey, et al., bioRxiv - Microbiology 2024

Quote: ... 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] ammonium salt (DSPE-PEG (2000) maleimide) (Avanti Polar Lipids, >99% purity) and cholesterol (Avanti Polar Lipids ...

Agnes Acevedo-Canabal, et al., bioRxiv - Pharmacology and Toxicology 2023

Quote: ... Gly5-ol]-Enkephalin (trifluoroacetate salt) was purchased from Tocris. For the in vitro studies ...

Natalie Bruiners, et al., bioRxiv - Immunology 2020

Quote: ... GGTI 298 trifluoroacetate salt hydrate and U18666A were purchased from Cayman Chemical Company (Ann Arbor MI) ...

Natacha Mariano, et al., bioRxiv - Biochemistry 2023

Quote: ... the cells were discarded and N-ethyl-maleimide (Merck, 2 mM) and Pefabloc (Roth ...

Chris H. Hill, et al., bioRxiv - Molecular Biology 2021

Quote: ... RNAs were labelled at the 5′ end with A647-maleimide or Cy5-maleimide conjugates (GE Healthcare) using the 5′ EndTag kit (Vector Labs ...

Isabel Soto, et al., bioRxiv - Neuroscience 2024

Quote: ... and aged (18-month-old) WT (n=10; (n=5 from Envigo and n=5 from Charles River) and KO (n=10 ...

Xu Zhao, et al., bioRxiv - Genomics 2021

Quote: ... After two washes with cold PBS the cells were collected in the Lysis buffer (5 mM PIPES pH 7.5, 85 mM KCl, 0.5% NP40, 20 mM N-ethyl maleimide [NEM] and protease inhibitor co*cktail [04693159001, Roche]) and incubated at 4°C for 10 minutes with rotation ...

Ayushi Verma, et al., bioRxiv - Cancer Biology 2021

Quote: ... XenoLight D-Luciferin potassium salt (P/N 122799) obtained from Perkin Elmer. ProLong™ Gold Antifade Mountant was purchased from Invitrogen ...

Sajishnu P Savya, et al., bioRxiv - Bioengineering 2022

Quote: ... n = 5) (Jackson Labs, Bar Harbor, ME). Acute studies (0 -6 h ...

Tristan A. Bell, et al., bioRxiv - Biochemistry 2023

Quote: ... 5% n-dodecyl-beta-maltoside (DDM) (Anatrace)) and incubated for 1 h at 4C with end-over-end rotation ...

Ta I Hung, et al., bioRxiv - Biophysics 2023

Quote: ... ISG15-AVTRYVDC was further crosslinked with fluorescein-5-Maleimide (Santa Cruz Biotechnology) at 4 °C for 1 hour ...

Michalis Amoiridis, et al., bioRxiv - Cell Biology 2023

Quote: ... 0.1% N-Lauroylsarcosine salt and 0.2% Triton-X100 and passed through Miniprep (QIAGEN) silica-fibre membrane spin columns in order to bind only the Top2cc’s and not DNA ...

Seung-won Choi, et al., bioRxiv - Plant Biology 2020

Quote: ... potassium salt (Promega) and left to stand for 5 min ...

Naoki Hayashi, et al., bioRxiv - Synthetic Biology 2021

Quote: ... carbenicillin disodium salt (Gold Biotechnology, 5 μg/mL), tetracycline hydrochloride (IBI Scientific ...

Bingqing Hao, Karen A. Beningo, bioRxiv - Cell Biology 2023

Quote: ... concentration was fixed at 5% while N,N-methylene-bis-acrylamide (bis, Bio-rad) varied from 0.04% to 0.1% to attain different stiffnesses of the substrates ...

Molly McPartland, et al., bioRxiv - Pharmacology and Toxicology 2023

Quote: ... We used 5’-N-Ethylcarboxamidoadenosine (NECA, Abcam, ab120440) and melatonin as positive controls for ADORA1 and MTNR1A ...

Isabel Soto, et al., bioRxiv - Neuroscience 2024

Quote: ... and aged (18-month-old) WT (n=10; (n=5 from Envigo and n=5 from Charles River) and KO (n=10 ...

Irina V. Mikheyeva, et al., bioRxiv - Microbiology 2023

Quote: ... cells were subjected first to hyperosmotic shock with 1 M sorbitol in LB and then treated with 5% N-lauroylsarcosine sodium salt (MP biomedicals, Cat. #190289) to remove the OM ...

Andrea Roeser, et al., bioRxiv - Neuroscience 2023

Quote: ... AAV9-hSyn- GrabDA1h (n = 5 Area X hemispheres; n = 2 MST hemispheres; Addgene), AAV5-CAG- Dlight1.1 (n = 1 Area X hemisphere ...

Motohiro Nonaka, et al., bioRxiv - Pharmacology and Toxicology 2020

Quote: ... wells of Sulfhydryl-BIND Surface Maleimide plates (Corning) were coated 20 hours with wild type (WT ...

Chen-Jun Guo, et al., bioRxiv - Biochemistry 2023

Quote: ... 5 mM ATP (Takara, sodium salt, pH 7.0), and 0.5 mM NADPH (Roche ...

Marzena Stefaniuk, et al., bioRxiv - Neuroscience 2021

Quote: ... binge-like drinking (BD group; n = 5), cue reexposure (CR group ...

Takamasa Kudo, et al., bioRxiv - Systems Biology 2023

Quote: ... with maleimide chemistry (Cell Signaling Technology #8478), Nos2 Alexa Fluor 532 conjugate (ThermoFisher Scientific ...

Katie M. Campbell, et al., bioRxiv - Genomics 2020

Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina, PT0017_Covaris_20ng_Illumina, Agilent_UHR_20ng_Illumina ...

Katie M. Campbell, et al., bioRxiv - Genomics 2020

Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina, PT0017_Covaris_20ng_Illumina, Agilent_UHR_20ng_Illumina ...

Evert den Drijver, et al., bioRxiv - Microbiology 2019

Quote: ... Both cefotaxime sodium salt and ceftazidime sodium salt were obtained from VWR International (Radnor ...

Aleksandra Boikova, et al., bioRxiv - Molecular Biology 2023

Quote: ... Reaction was set up using ARCA (NEB, S1411L or Trilink, N-7003-5) and N1-Methylpseudouridine-5’-Triphosphate (Trilink ...

Tatiana Lebedeva, et al., bioRxiv - Developmental Biology 2022

Quote: ... Red Sea Salt) in an optical bottom 35 mm Petri dish (D35-20-1.5-N, Cellvis, US) and imaged with a 20X CFI Plan Apo Lambda Objective (Nikon ...

Mariska S. Simpson, et al., bioRxiv - Cell Biology 2023

Quote: All peptides used for binding assays (Data S1) were synthesized with a N-terminal 5-carboxyfluorescien (5-FAM) at >85% purity (GenScript); peptides used for competition studies did not have 5-FAM ...

Sarah Körber, et al., bioRxiv - Cell Biology 2022

Quote: ... for TIRF-M imaging or with N-(1-Pyrenyl)maleimide (Invivogen) for pyrene-actin polymerization assays ...

C.W.E. Embregts, et al., bioRxiv - Immunology 2021

Quote: ... The virus was propagated in the human neuroblastoma cell line SK-N-SH in Eagle’s Minimum Essential Medium (EMEM) with Earle’s Balanced Salt Solution (EBSS) (Lonza), supplemented with 10% (v/v ...

Sung Ji Ahn, et al., bioRxiv - Neuroscience 2022

Quote: ... a map of the vasculature was taken through a ×5 objective (MPlan N 5□×□0.1 NA, Olympus) and compared with laser speckle map to locate the same imaging field in the whisker barrel cortex and identify pial vessels branches that feed the barrel cortex ...

Yueyang Wang, et al., bioRxiv - Cell Biology 2022

Quote: ... Images were acquired using an N-STORM/N-SIM TIRF microscope (Nikon) with a 1.49/60x Apo TIRF oil objective and a laser-scanning confocal microscope (LSM 800 ...

Ed Zandro M. Taroc, et al., bioRxiv - Developmental Biology 2019

Quote: ... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...

Shubhi Pandey, et al., bioRxiv - Biochemistry 2021

Quote: ... C5aR1−/− and C5aR2−/− mice on a C57BL/6J genetic background (n=5-15) were administered with recombinant mouse C5a (Sino Biological, China) at a dose of 50 μg kg-1 via intravenous injection (tail vein) ...

Bo Am Seo, et al., bioRxiv - Neuroscience 2024

Quote: ... 5 µg/ml of N-acetyl cysteine and 5 ng/ml HBEGF (#100-47, Peprotech) as previously described (Liddelow et al. ...

Priscilla N. Avalos, et al., bioRxiv - Cell Biology 2024

Quote: ... High salt solutions were transferred to Zymo RNA columns (Zymo RNA Clean & Concentrator 5 kit, R1013) for DNAse treatment and purification as per the manufacturer’s instructions.

Troy N. Trevino, et al., bioRxiv - Immunology 2022

Quote: ... 50,000 CD4+ T cells were added to each well of mBECs in 12-well chamber slides in Hank’s balanced salt solution (HBSS) with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...

Shivam A. Zaver, et al., bioRxiv - Cell Biology 2023

Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...

Stephanie S. Kim, et al., bioRxiv - Cancer Biology 2021

Quote: Cells were grown to 70% confluent in 24-well plates prior to treatment with N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89; R&D Systems) or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437 ...

Ning Zhang, et al., bioRxiv - Biochemistry 2019

Quote: ... Biotin maleimide was purchased from Vector Laboratories (Burlingame, CA, USA). Cyanine3 maleimide (Cy3 ...

Ming Bi, et al., bioRxiv - Systems Biology 2023

Quote: Permethylated N-glycans were trapped on a C18 (5 μm, 300 Å, Jupiter, Phenomenex) trap column and separated on a C18 analytical column (75 μid ...

Ana M Lyons, Kevin T Roberts, Caroline M Williams, bioRxiv - Physiology 2024

Quote: ... N = number of sample replicates (groups of n=∼20 tardigrades in a single Eppendorf tube). The majority of samples at -10°C remained unfrozen ...

Sabah Mozafari, et al., bioRxiv - Neuroscience 2020

Quote: ... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...

Maria L. White, et al., bioRxiv - Microbiology 2022

Quote: ... 5 (BioTek). OD600nm readings were taken every hour and growth curves were plotted using GraphPad Prism 9.

Amanda M. Hamilton, et al., bioRxiv - Cell Biology 2019

Quote: Viability of unlabeled and ferumoxytol-labeled murine MSCs (n=4) and human MSCs (n=3) was assessed by Trypan blue dye exclusion using ViCell (Beckman Coulter).

Paula García-Huerta, et al., bioRxiv - Neuroscience 2020

Quote: ... Alexa 594 goat anti rabbit 1.1000 (Jackson ImmunoResearch, cat n° 111-545-144) and DAPI 1:10,000 (Merck ...

Shintaro Shirahama, et al., bioRxiv - Immunology 2024

Quote: ... monosodium salt) using a Spectra Max M3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA).